The advantages of using Enhanced Autoradiography with EA-Wax have been fully evaluated in many real-world experimental situations. Full descriptions and autoradiographic images are available for viewing from this page. For your convenience, these experiments are also described in full in the EA Biotech Information Pack. You can request copies of this from EA Biotech or download an electronic version from this web site.

Click on the text link to view details of each of the experiments listed. Clicking on the images link will load a  large size image of the autoradiograms into a separate browser window. When you are finished viewing the image just close the browser window.

Experimental Evaluations

Separation of Tritiated(^3H) and Carbon-14(^14C) Monosaccharides	text	images
^3H-DNA Dot Blo	text	images
^3H-Methyltransferase Assa	text	images
^3H-labelled PCR product	text	images
Detection of ^3H on TLC plate	text	images
Western blot analysis of ^14C-labelled membrane protei	text	images
2D gel analysis of ^35S-methionine labelled proteins in S. typhimurium SL134	text	images
2D gel analysis of ^35S-methionine labelled proteins in Campylobacter jejuni	text	images
2D gel analysis of E.coli global protein change during GroELS depletion	text	images

Summary of the Benefits of Enhanced Autoradiography.

1. Tritium(³H) has always been the isotope of choice for both research, and routine laboratory use, but it has always been the most difficult to detect. Enhanced Autoradiography solves the detection problem. A revolution is now possible which will effect many branches of science.

2. Enhanced Autoradiography substantially reduces the time interval between an experiment or procedure being performed and the results being obtained.

3. The process is in general non-destructive of the sample, so further analysis can be performed on the same sample.

4. The invention allows the accurate quantitation of radioactivity particularly that of tritium(³H) which currently is not possible.

5. High cost equipment, with high cost consumables and maintenance are not required. Equipment found in most laboratories, an oven and refrigerator, are all that are required in addition to the EA-Wax. This of course means that Enhanced Autoradiography will open doors, to research and routine science, in the Third World countries as well as developed countries.

6. The materials used in Enhanced Autoradiography are low cost and environmentally friendly. They are also inherently safe to handle, store, transport and dispose of.

7. The final method of detection is X-ray film, which gives the highest possible resolution.